Electro-fabrication of Molecular Imprinted Polymer Biosensor: Efficient Approach to Achieve Reliable and Reproducible Biosensors

Abstract

In this investigation, a reagent-less and ultrasensitive electrochemical biosensors by stabilizing electrodeposited Prussian blue nanoparticles (PB NPs) as the embedded redox probe was designed based on the concept of molecularly imprinted polymers (MIP) as artificial receptors for highly sensitive and specific detection of agmatine. The MIP biosensor was fabricated by the electrodeposition of PB, electropolymerization of pyrrole (Py) in the presence of agmatine as a template molecule, and electro-cleaning of template molecules, which each electrosynthesis step of the MIP biosensors has been monitored by electrochemical approach. The presence of Prussian blue in the structure of the MIP biosensor provides the possibility of monitoring all the fabrication steps of the biosensor through electrochemical methods which leads to screening the electrodes during electrosynthesis of MIP biosensors that can be used as an efficient quality control (QC) approach to achieve reproducible MIP biosensors.