Innovative Strategy for Quality Control of Preparing Molecularly Imprinted Polymer Biosensor for Grail Fibrillary Acidic Protein Detection

Authors

  • Mohammadreza Farrokhnia 1-Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada, 2- Fluidome Company, Calgary, Alberta, Canada
  • Bahareh Babamiri 1-Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada, 2- Fluidome Company, Calgary, Alberta, Canada
  • Mehdi Mohammadi Ashani Fluidome Company, Calgary, Alberta, Canada
  • Amir Sanati-Nezhad 1 Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada

Keywords:

Quality Control, Molecularly Imprinted Polymer Biosensor, Grail Fibrillary Acidic Protein (GFAP), reproducibility, Electro-fabrication

Abstract

The reproducibility of biosensors is an important parameter for mass-production of biosensors in different applications. In this paper, the novel strategy for screening the electrodes has been introduced to improve the reliability and performance of fabricated molecularly imprinted polymer biosensors that is the main challenge of the almost research in this field. In this strategy, we just use the non-interfering quality controls during the fabrication procedures. By using electro-fabrication approach for each step of preparation and analyzing their results, we could make the perfect decision about screening of electrodes. According to this strategy, we could prepare reproducible biosensors for detection of grail fibrillary acidic protein (GFAP). As the bare screen-printed electrodes suffer from a lack of reproducibility because of variable resistance, capacitance or electroactive area, the percent of preparing the repetitive and good performance GFAP biosensors to bare electrodes is about 36 % (13 of 36). The impedance electrochemical results showed the efficient performance of this novel approach for preparing high quality MIP biosensors.

Author Biographies

Mohammadreza Farrokhnia, 1-Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada, 2- Fluidome Company, Calgary, Alberta, Canada

Dr. Mohammadreza Farrokhnia is a Mitacs Postdoctoral Associate in the department of Biomedical
Engineering at the University of Calgary and the Fluidome Company. He received his Ph.D. Degree in the
field of Biomedical Engineering from the College of Engineering, University of Tehran, Iran. His Ph.D.
thesis was a collaboration between the Research Center of New Technologies in Life Science Engineering
(UTLSE) at the University of Tehran, Iran, and the Nanomaterials and Polymer Nanocomposites
Laboratory (NPNL) at the University of British Columbia, Canada, focusing on the design, fabrication,
and development of multiplexed nano-biosensor system for the early detection of diabetes. He received
his M.Sc. Degree in Chemical Engineering/Separation Processes Design from the Sharif University of
Technology, Iran focused on the synthesis and investigation of various polymeric nanocomposite
membranes and B.Sc. Degree in Chemical Engineering from Shiraz University, Iran.

Bahareh Babamiri, 1-Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada, 2- Fluidome Company, Calgary, Alberta, Canada

Dr. Bahareh Babamiri received her B.Sc., M.Sc., and Ph.D. in Analytical Chemistry-Electrochemistry from Kurdistan University in Iran. Bahareh specializes in electrochemiluminescence, electrochemical and fluorescence biosensors for trace detection of proteins, tumor biomarkers, bacteria, DNA, and mRNA toward their application in clinical diagnostics. Her Ph.D. thesis project was the design and development of nano-biosensors for the simultaneous determination of highly important tumor biomarkers, Aptamer-based biosensors, DNA and RNA hybridization biosensors, and molecularly imprinted polymer (MIP) sensors based on spectro-electrochemical techniques. 

Currently, Bahareh is a Mitacs postdoctoral fellow at the Department of Mechanical Engineering, and her current research focuses on developing point-of-care dd-PCR kits and biosensors for rapid diagnosis of bacteria and viral infections.

Mehdi Mohammadi Ashani, Fluidome Company, Calgary, Alberta, Canada

I hold a Ph.D. and serve as a Senior Scientist, Biomedical Device Entrepreneur, and Senior Mechanical and Microfluidic Engineer. My expertise spans microbiology and metabolomics for rapid diagnostics, and my passion lies in translational research, transitioning from invention to innovation to make a tangible impact on patients' lives. With 18 years of multidisciplinary experience, I've explored new frontiers in engineering, translational medicine, and science. In 2020, leveraging my extensive background, I founded Fluidome with a mission to revolutionize rapid diagnostic tools and knowledge.

As the President and CEO of Fluidome, my role involves shaping and communicating the company's vision and mission. Additionally, I lead technical directions and foster strategic partnerships. Over the years, I've successfully developed and directed more than 25 microfluidic, metabolomics, and biomedical point-of-care projects, contributing valuable insights to the field. My commitment to advancing knowledge is reflected in the publication of over 45 peer-reviewed scientific research articles. My journey is defined by a relentless pursuit of translating scientific discoveries into practical solutions for the benefit of healthcare and society.

Amir Sanati-Nezhad, 1 Department of Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada

Dr. Amir Sanati-Nezhad is an Associate Professor of the Department of Biomedical Engineering and the Department of Mechanical and Manufacturing Engineering with a joint affiliation with the Center of BioEngineering for Research and Education (CBRE), at the Schulich School of Engineering, University of Calgary. He is also the Principal Investigator of BioMEMS and Bioinspired Microfluidic Laboratory. Amir completed his Postdoctoral trainings at the Harvard and McGill Universities (2013-2015), and accomplished his PhD program at the Concordia University (2010-2013). His research program has the focus on bioinspired microfluidics, tissue engineering and biosensors. Dr. Sanati-Nezhad has published over 250 peer-reviewed journal articles, book chapters, and conference papers. He has also presented several invited talks in national and international conferences. Dr. Sanati has received numerous prestigious awards and recognitions during his research career, including: "CMC Microsystem's Douglas R. Colton Medal for Research Excellence" and "Governor's General Gold Medal of Canada", and "Canada Research Chair".

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Published

2024-06-26

How to Cite

[1]
M. Farrokhnia, B. Babamiri, M. . Mohammadi Ashani, and A. Sanati-Nezhad, “Innovative Strategy for Quality Control of Preparing Molecularly Imprinted Polymer Biosensor for Grail Fibrillary Acidic Protein Detection”, CMBES Proc., vol. 46, Jun. 2024.

Issue

Vol. 46 (2024)

Section

Academic