A 3D-printed model of the subarachnoid space to study the role of meningothelial cells during optic nerve compartmentalization

Abstract

The cerebrospinal fluid (CSF)-filled subarachnoid space (SAS) between the arachnoid and pia mater is lined by meningothelial cells (MECs) and are in direct contact with flowing CSF. MECs are thought to contribute to maintaining CSF equilibrium by secreting proteins and eliminating neurotoxic waste via endocytosis. As CSF homeostasis is critical for neuronal function, MECs are deemed neuroprotective. Therefore to study MEC metabolism during optic nerve compartment syndrome, we developed a 3D-printed, perfusable SAS model compatible with confocal microscopy under (patho)physiological flow conditions.