In-Vitro Characterization of APA Microcapsules for Oral Delivery of Live Bacterial Cells

Authors

  • Hongmei Chen McGill University
  • Wei Ouyang McGill University
  • Mitchell Jones McGill University
  • Tashima Hasque McGill University
  • Bisi Lawuyi McGill University
  • Aleksandra Urbanska McGill University
  • Satya Prakash McGill University

Abstract

The suitability of the alginate-poly-L-lysine-alginate (APA) microcapsules for gastrointestinal (GI) applications was evaluated using a simulated human GI microbial ecosystem (SHIME). The viability of the encapsulated Lactobacillus cells in simulated gastric solutions (pH=1.2 and 2.0) was investigated. Results shows that the APA membrane was morphologically stable in the simulated stomach. However, when exposed to the simulated GI system for 3 days, it was unable to retain integrity. Studies of cell resistance to gastric acidity also demonstrated the limitations of the currently obtainable APA membrane for live bacterial cell oral delivery. The APA microcapsule system may, therefore, require significant improvement to withstand biological impediments and ensure adequate protection of the live cells when used orally.

Author Biographies

Hongmei Chen, McGill University

Department of Biomedical Engineering and Physiology,

Faculty of Medicine

Wei Ouyang, McGill University

Department of Biomedical Engineering and Physiology,

Faculty of Medicine

Mitchell Jones, McGill University

Department of Biomedical Engineering and Physiology,

Faculty of Medicine

Tashima Hasque, McGill University

Department of Biomedical Engineering and Physiology,

Faculty of Medicine

Bisi Lawuyi, McGill University

Department of Biomedical Engineering and Physiology,

Faculty of Medicine

Aleksandra Urbanska, McGill University

Department of Biomedical Engineering and Physiology,

Faculty of Medicine

Satya Prakash, McGill University

Department of Biomedical Engineering and Physiology,

Faculty of Medicine

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Published

2005-12-31

How to Cite

[1]
H. Chen, “In-Vitro Characterization of APA Microcapsules for Oral Delivery of Live Bacterial Cells”, CMBES Proc., vol. 28, no. 1, Dec. 2005.

Issue

Section

Academic