In-Vitro Characterization of APA Microcapsules for Oral Delivery of Live Bacterial Cells
Abstract
The suitability of the alginate-poly-L-lysine-alginate (APA) microcapsules for gastrointestinal (GI) applications was evaluated using a simulated human GI microbial ecosystem (SHIME). The viability of the encapsulated Lactobacillus cells in simulated gastric solutions (pH=1.2 and 2.0) was investigated. Results shows that the APA membrane was morphologically stable in the simulated stomach. However, when exposed to the simulated GI system for 3 days, it was unable to retain integrity. Studies of cell resistance to gastric acidity also demonstrated the limitations of the currently obtainable APA membrane for live bacterial cell oral delivery. The APA microcapsule system may, therefore, require significant improvement to withstand biological impediments and ensure adequate protection of the live cells when used orally.Downloads
Published
2005-12-31
How to Cite
[1]
H. Chen, “In-Vitro Characterization of APA Microcapsules for Oral Delivery of Live Bacterial Cells”, CMBES Proc., vol. 28, no. 1, Dec. 2005.
Issue
Section
Academic