In-Vitro Characterization of APA Microcapsules for Oral Delivery of Live Bacterial Cells

Hongmei Chen; Wei Ouyang; Mitchell Jones; Tashima Hasque; Bisi Lawuyi; Aleksandra Urbanska; Satya Prakash

Authors

Hongmei Chen McGill University
Wei Ouyang McGill University
Mitchell Jones McGill University
Tashima Hasque McGill University
Bisi Lawuyi McGill University
Aleksandra Urbanska McGill University
Satya Prakash McGill University

Abstract

The suitability of the alginate-poly-L-lysine-alginate (APA) microcapsules for gastrointestinal (GI) applications was evaluated using a simulated human GI microbial ecosystem (SHIME). The viability of the encapsulated Lactobacillus cells in simulated gastric solutions (pH=1.2 and 2.0) was investigated. Results shows that the APA membrane was morphologically stable in the simulated stomach. However, when exposed to the simulated GI system for 3 days, it was unable to retain integrity. Studies of cell resistance to gastric acidity also demonstrated the limitations of the currently obtainable APA membrane for live bacterial cell oral delivery. The APA microcapsule system may, therefore, require significant improvement to withstand biological impediments and ensure adequate protection of the live cells when used orally.