Metabolic Reprogramming of Host Cells Induced by Cancer Cells: Implications for Premetastatic Niche Formation

Abstract

The effect of cell-cell interactions in promoting metabolic alterations and transition of normal fibroblasts to CAFs were investigated using the MIMETAS OrganoPlate 3-lane 40 platform, revealing a significant decrease in Gln concentration, and increase in L-glutamic acid production by MRC5 cells following cancer cell-lung cell crosstalk. The observed metabolic shift suggests the potential transition of normal lung fibroblasts towards a CAF phenotype, indicating their contribution to creating a favorable environment for hosting cancer cells before their arrival.